KM8P培養基怎么滅菌?

KM8P培養基怎么滅菌?

使用壓力蒸汽滅菌器120℃滅菌30分鐘，取出冷卻
KM8P培養基成分復雜，配方如下。

Ammonium Nitrate               600.0 mg/L
Boric Acid                      3.0 mg/L
Calcium Chloride, Anhydrous     453.0 mg/L
Cobalt Chloride 6H2O            0.025 mg/L
Cupric Sulfate 7H2O             0.025 mg/L
Na2 EDTA                        37.25 mg/L
Ferrous Sulfate 7H2O            27.85 mg/L
Magnesium Sulfate               146.55 mg/L
Manganese Sulfate H2O           10.0 mg/L
Molybdic Acid, Sodium Salt H2O  0.25 mg/L
Potassium Chloride               300.0 mg/L
Potassium Iodide                 0.75 mg/L
Potassium Nitrate                1900.0 mg/L
Potassium Phosphate, Monobasic   170.0 mg/L
Zinc Sulfate 7H2O                 2.0 mg/L
p-Aminobenzoic Acid              0.02 mg/L
L-Ascorbic                       2.0 mg/L
Biotin                          0.01 mg/L
D-Calcium Pantothenate          1.0 mg/L
Choline Chloride                1.0 mg/L
Folic Acid                      0.4 mg/L
myo-Inositol                    100 mg/L
Nicotinamide                    1.0 mg/L
Pyridoxine HCl                  1.0 mg/L
Riboflavin                      0.2 mg/L
Thiamine HCl                    1.0 mg/L
Vitamin A                       0.01 mg/L
Vitamin B12                     0.02 mg/L
其中的以下類別是不能使用壓力蒸汽滅菌器來滅菌的：vitamino 是維生素類，Cobalt Chloride 6H2O酮類衍生物，Cupric Sulfate 7H2O 7水合硫酸亞鐵，p-Aminobenzoic Acid 對氨基苯甲酸，L-Ascorbic 左旋vc，Biotin生物素，D-Calcium Pantothenate，d型泛酸鈣，Choline Chloride氯化膽堿，Folic Acid 葉酸，Nicotinamide煙酰胺，Pyridoxine HCl鹽酸吡多辛（vb6），Riboflavin核黃素，Thiamine HCl 硫胺素。